A Laboratory Manual of Analytical Methods of Protein Chemistry (Including Polypeptides), Volume 1: The Separation and Isolation of Proteins deals with the techniques used in the separation and isolation of proteins, including fractionation and characterization by dialysis, multi-membrane electrodecantation, and zonal density gradient electrophoresis. The fractionation of proteins by adsorption and ion exchange is also described. This book is comprised of seven chapters and begins with a discussion on procedures for the separation of proteins, paying particular attention to the liberation of proteins from cellular material; removal of lipids from lipoproteins; and denaturation, fractionation, and purification of proteins. The next chapter focuses on the isolation of biologically active proteins such as cytochrome, bacterial amylases, and bacterial proteinases. The reader is methodically introduced to fractionation of proteins by adsorption and ion exchange; fractionation and characterization by dialysis; multi-membrane electrodecantation; and continuous and discontinuous partition. The final chapter explains how zonal density gradient electrophoresis works as a separation method for natural mixtures of proteins, their degradation products, and other substances carrying electric charges in solution or suspension. This volume will be of interest to chemists working with proteins.a Laboratory Manual of Analytical Methods of Protein Chemistry (Including Polypeptides) P. Alexander, R. J. Block ... of the yeast lactic dehydrogenase present in the crude sample by an immediate centrifugation (6000 x g, for 10 min) . ... The complete oxidation is easily confirmed by disappearance of the o- absorption spectrum at 550 mu of reduced cytochrome-c under a microspectroscopic observation.
|Title||:||The Separation and Isolation of Proteins|
|Author||:||P. Alexander, R. J. Block|
|Publisher||:||Elsevier - 2014-05-15|