The interaction between phloem feeding aphids Aphis gossypii (cotton-melon aphid) and Cucumis melo (melon) provides an opportunity to elucidate the means by which a host plant defends itself against aphid attack. The relationship is of scientific as well as economic importance since A. gossypii is among the most destructive insects to agriculture in the U.S. and abroad. The resistance to A. gossypii in melon is controlled at the Vat (Vabarbelow;irus aabarbelow;phid tabarbelow;ransmission) locus by a major dominant gene, Vat that has the unique feature of conferring resistance to A. gossypii and resistance to transmission of non-persistent viruses by A. gossypii. The genetic and physical characterization of the Vat gene and its product supports a model of a resistance (R)-gene mediated resistance against A. gossypii that is similar to plant resistance against a wide range of pathogenic organisms. However, the specific recognition, signaling pathways, and proximate causes of resistance against this aphid are unknown. The study hypothesizes that Vat mediated-responses are specific to resistant AR 5(Vat+) plants differing from the basal defense or physiological responses to aphid feeding occurring in susceptible PMR 5 (Vat-) plants and provide physiological and morphological attributes that can counter A. gossypii infestation.Sequencing was evaluated for the quality parameters indicative of reliable sequence as specified by the CEQ 2000XL manual (Beckman Coulter). Removal of vector sequences and trimming of low quality was performed manually. Trimmedanbsp;...
|Title||:||Transcriptional Profiling of Aphid Resistant and Susceptible Melon (Cucumis Melo) Following Cotton-melon Aphid (Aphis Gossypii) Feeding|
|Publisher||:||ProQuest - 2008|